vimentin concentrations (Cusabio)
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vimentin concentrations
Vimentin Concentrations, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vimentin+concentrations/VIM/pm22978525-89-1-19
Average 92 stars, based on 3 article reviews
Vimentin Concentrations, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vimentin+concentrations/VIM/pm22978525-89-1-19
Average 92 stars, based on 3 article reviews
vimentin concentrations - by Bioz Stars,
2026-09
92/100 stars
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1) Product Images from "A novel compound, denosomin, ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin."
Article Title: A novel compound, denosomin, ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin.
Journal: British journal of pharmacology
doi: 10.1111/j.1476-5381.2012.02211.x
Figure Legend Snippet: Figure 7 Denosomin-mediated axonal outgrowth in primary cultures is mediated by vimentin secretion from astrocytes. (A–C) Isolated astrocytes were cultured in the presence or absence of 1 mM denosomin (Pre-Deno) for 6 days. Rat spinal cord cells were cocultured on the astrocyte layer without denosomin for 7 days after the astrocytes had been treated with denosomin. (D–F) Isolated astrocytes were cultured in the presence or absence of 1 mM denosomin (Pre-Deno) for 6 days. The culture medium was then replaced with fresh medium without denosomin, and the medium was collected 24 h later for use as astrocyte-conditioned medium (ACM). Rat primary cultured spinal cord cells were cultured in normal medium or ACM for 6 days (F). (G and H) Cultured astrocytes that were treated with or without denosomin (Deno) (G) and ACM collected from the astrocyte culture (H) were used for the ELISA assay. (I–K) Isolated mouse cortical cells were cultured with or without 1 or 10 ng·mL-1 vimentin for 6 days. The cells were cultured on slides with (J and K) or without (I–J) a CSPG coating. The cells were immunostained for pNF-H and MAP2, and the densities of pNF-H-positive axons on each neuron were quantified (B, D, I and K). Cellular distributions of neurons and non-neuronal cells per 0.1 mm2 were evaluated by the quantification of MAP2- and DAPI-positive cells (E). In (B, G and H): *P < 0.05, Student’s unpaired t-test (two-tailed); #P < 0.05; one-way ANOVA followed by the post hoc Bonferroni test, versus the control ACM treatment (D), versus the control cells (I) or versus CSPG-coated control cells (K). The numbers of photos (B, D, E, I, and K) or measurements (G and H) are shown in parentheses in the columns. The scale bar indicates 100 mm.
Techniques Used: Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Control
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Enzyme-linked Immunosorbent Assay:Article Title: A novel compound, denosomin, ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin Article Snippet: The cells were lysed using a lysis buffer (pH 7.5, 140 mM NaCl, 50 mM Tris–HCl, 1% NP-40, 10 mM EDTA, 20 mM NaF, 20 mM β-glycerophosphate, 1 mM PMSF, 1 mM DTT, 1 mM sodium vanadate and a protease inhibitor cocktail; Roche). .. The Article Title: A novel compound, denosomin, ameliorates spinal cord injury via axonal growth associated with astrocyte-secreted vimentin. Article Snippet: The cells were lysed using a lysis buffer (pH 7.5, 140 mM NaCl, 50 mM Tris–HCl, 1% NP-40, 10 mM EDTA, 20 mM NaF, 20 mM b-glycerophosphate, 1 mM PMSF, 1 mM DTT, 1 mM sodium vanadate and a protease inhibitor cocktail; Roche). .. The |
